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1)  suppression subtractive hybridization
抑制消减杂交
1.
Suppression subtractive hybridization coupled with gene chip technologies and their application;
抑制消减杂交与基因芯片技术及其联合应用
2.
Cloning of differentially expressed genes of eosinophils from asthmatic patients by suppression subtractive hybridization;
应用抑制消减杂交克隆支气管哮喘病人嗜酸细胞差异表达基因
3.
Suppression subtractive hybridization in construction of radiation-induced EST library;
运用抑制消减杂交构建辐射诱导EST库
2)  SSH
抑制消减杂交
1.
Construction and Analysis of Differentially Expressed Genes Libraries of Microsporum Canis Incubated in Different Induced Medium by Using SSH
利用抑制消减杂交初步构建犬小孢子菌在不同诱导底物表达的差异基因文库
2.
Then suppression subtractive hybridization (SSH) was performed on control HUVEC versus activated HUVEC.
利用抑制消减杂交 (SSH)获得活化HUVEC高表达的基因片段 ,放射标记后筛选文库 。
3.
Methods Suppression subtractive hybridization(SSH) and cDNA microarray were performed.
方法 抑制消减杂交 (SSH) ,cDNA芯片。
3)  suppression subtractive hybridization (SSH)
抑制消减杂交
1.
Two cDNA libraries enriched for transcripts differentially expressed in plants of two rice lines with similar genetic backgrounds and same leaf blast resistance but different responses to neck blast using suppression subtractive hybridization (SSH).
以遗传背景相近、对叶瘟抗性相同但对穗瘟抗性不同的两个水稻株系为材料,利用抑制消减杂交(SSH) 技术构建穗瘟抗/感消减cDNA文库,经差异筛选及序列分析,共获得90个独立的差异表达cDNA克隆,根据与它们同源的基因功能推测,这些克隆可能参与了对病原菌的防卫反应、信号传导和转录等一些重要的生物学过程。
2.
A fragment which is higher expression in the leaves of rice was isolated using suppression subtractive hybridization (SSH).
根据抑制消减杂交(suppressionsubtractivehybridization,SSH)方法分离到在水稻叶片中高表达的蔗糖转化酶基因片段,根据水稻基因组顺序设计引物分离到全长cDNA。
3.
In the present study, some cDNAs of the specially expressing genes of Dunaliella salina under hyperosmotic shock were cloned by suppression subtractive hybridization (SSH) and differentially screening of subtracted library.
抑制消减杂交及差式库的筛选对盐藻(Dunaliella salina)在高渗胁迫下特异表达基因片段进行了克隆。
4)  suppression subtractive hybridization(SSH)
抑制消减杂交
1.
Application of suppression subtractive hybridization(SSH) to study of cold tolerance in common carp(Cyprinus carpio);
抑制消减杂交技术在鲤鱼抗寒研究中的应用
2.
Differentially expressed cDNAs subtracted female from male gametophytes of Laminaria japonica were obtained by suppression subtractive hybridization(SSH).
利用抑制消减杂交法(suppression subtractive hybridization,SSH)获得了海带雌、雄配子体差异表达的cDNA片段,将雄配子体差异表达的cDNA片段克隆于pGEM-T载体并转化大肠杆菌(Escherichia coli)JM109,构建雄配子体差异表达的cDNA消减文库。
3.
The subtractive cDNA libraries for RH strain(typeⅠ) and QHO strain(typeⅡ) were constructed by the technique of suppression subtractive hybridization(SSH) using the PCR-Select cDNA Subtraction Kit.
抑制消减杂交技术(SSH)构建弓形虫强弱毒株正向消减(弱毒株消减强毒株)及反向消减(强毒株消减弱毒株)cDNA文库。
5)  suppressive subtractive hybridization
抑制消减杂交
1.
The suppressive subtractive cDNA library was set up using suppressive subtractive hybridization(SSH).
方法提取胰腺癌和癌旁正常胰腺组织中的RNA和mRNA,应用抑制消减杂交技术(SSH)构建消减cDNA文库。
6)  suppression subtractive hybridization
抑制性消减杂交
1.
Screening and cloning of target genes transactivated by human gene C1 encoding protein 1 interacting with HBV core antigen using suppression subtractive hybridization technique;
抑制性消减杂交技术筛选乙型肝炎病毒核心蛋白相互作用蛋白编码基因C1的反式调节基因
2.
Construction of cDNA library of hepatitis B virus with X protein C-terminally truncated 40 amino acids by suppression subtractive hybridization method;
抑制性消减杂交构建乙型肝炎病毒X蛋白羧基端缺失40个氨基酸cDNA文库
3.
Identification of genes associated with the metastasis of adenoid cystic carcinoma by suppression subtractive hybridization;
应用抑制性消减杂交技术分析涎腺腺样囊性癌转移相关基因
补充资料:抑制杂交翻译
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性质:又称杂交扣留翻译。能使一克隆DNA与它所编码的蛋白质相关联的方法。这个方法基于下述事实:以RNA在与它的DNA互补体杂交后就不会在无细胞的翻译系统中指导蛋白质的合成。无细胞系统所合成的蛋白质,包括一个特定的杂交mRNA,就能与未发生同DNA杂交的系统所合成的蛋白质相比较,其差别就表示了由所研究的mRNA编码的蛋白质的性质。

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