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1)  alkaline pectinase
碱性果胶酶
1.
The cultivation of alkaline pectinase strains and the application in fabric pretreatment;
碱性果胶酶菌种选育及在纺织前处理中的应用研究
2.
Research on the enzymatic degradation products from cotton knitwear scoured with alkaline pectinase;
棉针织物碱性果胶酶精练时酶解产物的研究
3.
Stability of alkaline pectinase applied in cotton fabric scouring;
纺织精练中碱性果胶酶稳定性的改进研究
2)  alkaline pectate lyase
碱性果胶裂解酶
1.
Production conditions of alkaline pectate lyase by alkaline microorganism Bacillus subtilis;
碱性果胶裂解酶摇瓶发酵条件的研究
2.
Based on time courses of the specific cell growth rate and specific alkaline pectate lyase production rate, a two-stage agitation rate control strategy was developed, in which agitation rate was controlled at 500 r/min at the first 5 h, then switched to 600 r/min afterwards.
在5L小型发酵罐上,考察了不同搅拌转速对细胞生长和碱性果胶裂解酶生产的影响。
3)  Pectate lyase
碱性果胶酯裂解酶
1.
The structure gene PL from Bacillus subtilis WSHB04-02 encoding pectate lyase was amplified by PCR method.
从本研究室筛选的BacillussubtilisWSHB04-02菌株中扩增出编码碱性果胶酯裂解酶的结构基因PL,将其插入大肠杆菌分泌表达载体pET22b(+)多克隆位点,得到重组载体pET22b(+)PL。
4)  alkaline pectate lyase
碱性果胶酯裂解酶
1.
Properties of alkaline pectate lyase from recombinant strain E.coli JM109(pHsh PL);
重组菌产碱性果胶酯裂解酶酶学性质研究
2.
Three factors for producing alkaline pectate lyase (PL) by recombinant strain Pichia pastoris: initial concentration of CH3OH, adding amount of CH3OH and concentration of yeast nitrogen base (YNB), were investigated.
31%,优化后碱性果胶酯裂解酶(PL)表达量达到40。
5)  homemade alkaline pectinase
国产碱性果胶酶精练
6)  thermostable pectate lyase
耐热碱性果胶裂解酶
1.
Expression, purification and characterization of a thermostable pectate lyase from Thermotoga maritima
海栖热袍杆菌来源的极耐热碱性果胶裂解酶的表达、纯化及定性
补充资料:果胶酶
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性质:催化植物细胞间质(即果胶质)水解成半乳糖醛酸和果胶酸的一类酶。按其作用方式,大致可分为三类:(1)原果胶酶(protopectinase),可使天然果胶转变为可溶性果胶。(2)果胶聚半乳糖醛酸酶(pectin polygalacturonase;PG),使果胶或果胶酸中α-1,4键分解,而生成半乳糖醛酸。(3)果胶甲酯水解酶(pectin methylesterase;PE),使果胶中甲酯水解,生成果胶酸。为使果胶完全分解,需要先用PE,再用PG。在果汁澄清中,亦同时用这两种酶。灰白色粉末或中黄色液体。最适作用pH值3.5~4.5,最适作用温度40~50℃。果胶酶广泛存在于植物和微生物中,动物细胞通常不能合成这类酶。工业上主要采用曲霉菌,如黑曲霉或青霉菌等生产。用于果汁加工(澄清果汁)、橘子脱囊衣以及制造浓缩果汁、果粉和低糖果冻。也可用于加强果酒的澄清效果,提高酒的收率。还可用于麻料脱胶和木材防腐等。

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