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1)  plasmid expression vector
质粒表达载体
1.
Construction and screening of plasmid expression vectors encoding the short hairpin RNA targeting hVEGF_(165) gene;
靶向hVEGF_(165)基因shRNA质粒表达载体的构建及筛选
2.
Methods: Construct pSilencerHSF1siRNA plasmid expression vector, plasmid expression vector which could express siRNA was transfected into cervical carcinoma cells by lipofectamine.
方法:构建pSilencerHSF1siRNA质粒表达载体,将构建好的质粒表达载体用脂质体转染入Hela细胞,实时荧光定量PCR检测HSF1 mRNA表达水平变化,用流式细胞仪检测HSF1蛋白表达的变化。
2)  Plasmid-vector for siRNA
siRNA质粒表达载体
1.
Plasmid-vector for siRNA was constructed and transfected into ameloblastoma cells.
方法:培养成釉细胞瘤细胞,应用免疫组织化学方法检测成釉细胞瘤中MMP-2的表达;体外构建MMP-2靶向siRNA质粒表达载体,将siRNA质粒表达载体转染原代培养的成釉细胞瘤细胞,激光共聚焦显微镜检测siRNA质粒表达载体的转染效率,RT-PCR检测成釉细胞瘤细胞中MMP-2mRNA的改变,明胶酶谱法检测MMP-2的变化,应用SPSS11。
3)  Lactococcus plasmid expression vector pNZ2103
乳酸菌质粒表达载体pNZ2103
4)  expression vector
表达质粒
1.
PurposeThe expression vector pTYB102 was constructed and active expression of nattokinase gene in E.
目的构建大肠杆菌表达质粒pTYB10 2 ,实现纳豆激酶基因 (nattokinasegene)在大肠杆菌中高活性表达。
2.
Recombinant expression vector was constructed and sequenced after enzyme digestion.
方法 :采用RT -PCR技术 ,从正常人外周血单个核细胞中扩增编码CD1 5 8b的cDNA ,经酶切后将其克隆于pMBP -c表达质粒上 ,酶切和测序鉴定。
3.
The full-length cDNA fragment encoding human C1-INH was obtained by gene recombination techniques and a stable expression plasmid was constructed by inserting the human C1-INH cDNA into an efficient dicistronic expression vector pED.
利用基因工程手段获取编码人补体1抑制物(C1-inhibitor,C1-INH)的基因片段,将其插入双顺反子表达载体pED中,构建成功可在CHO细胞中有效表达人C1-INH的表达质粒。
5)  Expression plasmid
表达质粒
1.
Construction and identification of siRNA expression plasmid aimed at UL49 gene of Marek′s disease virus RB1B strain;
马立克氏病病毒超强毒UL49基因siRNA表达质粒的构建与鉴定
2.
Sequence analysis and eukaryotic expression plasmid constructionof gE gene of pseudorabies virus strain MinA;
伪狂犬病病毒Min-A株gE基因序列分析及其真核表达质粒的构建
3.
Here, a expression plasmid that suitable for Agrobacterium-mediated transformation pUNDV was constructed, which carried fussion protein gene of Newcastle disease virus(NDV-F) under the control of ubiquitin(Ubi) promoter, the selectable marker hygromycin phosphotranferase gene(HPT) and the reporter glucuronidase gene(GUS).
以编码新城疫病毒融合蛋白(NDV-F)的基因为外源基因,以玉米泛素蛋白(Ubi)启动子为启动子熏以潮霉素磷酸转移酶穴HPT雪基因作为选择标记基因熏β-半乳糖苷酸酶(GUS)基因作为报告基因构建了适宜于农杆菌介导转化水稻的表达质粒pUNDV,并通过农杆菌介导转化水稻,获得了多株转基因植株。
6)  plasmid expression
质粒表达
补充资料:PUC18质粒DNA
CAS: 9007-49-2

中文名称: PUC18质粒脱氧核糖核酸;PUC18质粒DNA

英文名称: Deoxyribonucleic-acid;Calf thymus DNA;Desoxyribose nucleic acid;DNA;Nucleic acids, deoxyribo;deoxyribonucleic ac.;Nucleic acids,deoxyribo

说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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