1) Indirect immunofluorescence
间接免疫荧光
1.
Evaluation on the application of indirect immunofluorescence on salt split skin in differential diagnosis of bullous pemphigoid;
盐裂间接免疫荧光技术在大疱性类天疱疮鉴别诊断中的应用研究
2.
Effect of frozen section to indirect immunofluorescence with Different Fixatives
不同固定方法对冰冻切片间接免疫荧光结果的影响
3.
[Methods]Indirect immunofluorescence was used to detect IgM of respiratory tract pathogen in the serum of 80 samples.
[方法]应用间接免疫荧光技术检测80例呼吸道感染的患儿血清中呼吸道病原的IgM抗体,以调查小儿呼吸道病原体感染情况和流行特点。
2) IFA
间接免疫荧光
1.
Results 1 TCID_50 could be acquired with IFA,however,only 100 TCID_50 could be acquired with the other two .
结论间接免疫荧光检测方法比其它两种方法敏感性更高。
2.
Objective To establish indirect immunofluorescence assay(IFA) method for mycoplasm genitalium(Mg) antigen detection using monoclonal antibodies specific to Mg and evaluate its value in clinical application.
目的研究间接免疫荧光技术(IFA)检测生殖支原体(Mg)抗原的敏感性及特异性,并探讨其临床价值。
3.
Polyclonal antisera prepared by immunizing rabbits with rNP were employed to detect Hantavirus antigen in murine lungs through indirect fluorescence assay(IFA).
coliBL21(DE3)/pET32a-L99S经IPTG诱导表达rNP,采用Ni亲和层析方法纯化,纯化蛋白免疫日本大耳白兔,获取抗血清用于间接免疫荧光法(IFA)检测鼠肺组织的HV抗原;并与病人混合血清作为一抗的IFA结果进行比较,部分标本进一步用RT-PCR法进行验证。
3) Indirect immunofluorescence assay
间接免疫荧光
1.
Comparision between indirect immunofluorescence assay and in situ hybridization assay in detecting the expression of Bcl-2 and Bcl-2/Bax in acute leukemia;
间接免疫荧光法和原位杂交法检测急性白血病中Bcl-2和Bcl-2/Bax表达的对比研究
2.
Clinical application of indirect immunofluorescence assay to detect measles virus antigen in early diagnosis;
间接免疫荧光法检测麻疹抗原在麻疹早期诊断中的临床应用
3.
Methods Expression of E-cadherin in preimplantation embryos and blastocysts was detected by indirect immunofluorescence assay and quantitated by laser scanning confocal microscopy.
方法 应用特异性单克隆抗体 ,采用间接免疫荧光方法 ,检测人种植前胚胎 (分别为 2、4、6、7、8细胞胚及囊胚 )上皮型钙粘附蛋白的表达 ,并应用激光共聚焦显微镜对荧光进行定量检测。
5) Indirect immunofluorescence
间接免疫荧光法
1.
Application of indirect immunofluorescence(IFL) in the detect of antibody to eberthella typhi and bacillus paratyphosus;
间接免疫荧光法(IFL)测抗伤寒、副伤寒沙门菌IgM抗体
2.
Methods: The ANA was detected with indirect immunofluorescence(IIF) and protein chip respectively,and the other six autoantibodies including anti-dsDNA,anti-Ro-60/SSA,anti-Ro-52/SSA,anti-SSB,anti-Jo-1,and anti-phospholipid IgG were simultaneously detected with ELISA and protein chip technology,and numbers of the both positive,both negative and the si.
方法用酶联免疫吸附试验(EL ISA)、蛋白芯片法检测抗dsDNA、抗R o-60/SSA、抗R o-52/SSA、抗SSB、抗Jo-1、抗磷脂IgG抗体,用间接免疫荧光法(IIF)、蛋白芯片法检测抗核抗体(ANA),分别统计两种方法检测均阳性、均阴性和单阳性的标本数目,并进行评价。
3.
METHODS: Balb/c mice was immunized with pig cardiac myosin and complete freund ,s adjuvant(CFA), β 1 integrin on myocardium were analyzed by laser confocal microscopy and indirect immunofluorescence at the day 14,30,60 after the first immunization.
方法:猪肌凝蛋白皮下注射免疫Balb/c小鼠,建立慢性自身免疫性心肌炎模型,于初次免疫后14、30和60d对心肌组织进行病理学检查,同时用激光共聚焦技术和间接免疫荧光法检测整合素β1在心肌的表达变化。
6) indirect immunofluorescence(IIF)
间接免疫荧光法
1.
Objective To found the indirect immunofluorescence(IIF) of detecting immunoglobulin binding protein(Bip) antibody with Hela cells as antigenic sources and to provide the means for exploring the diagnostic value of Bip antibody in patients with rheumatoid arthritis(RA).
目的建立抗免疫球蛋白结合蛋白(Bip)抗体检测的间接免疫荧光法(IIF),为探讨其在类风湿关节炎(RA)中的意义做出方法学准备。
2.
Antinuclear antibodies(ANA)、antimitochondrial antibody(AMA) and anti-smooth muscle antibody(SMA) were tested by indirect immunofluorescence(IIF);Antibodies to soluble liver antigen/liver pancreas(SLA/LP)、l.
采用间接免疫荧光法(IIF)检测各组抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA);采用免疫印迹法检测抗可溶性肝抗原/肝胰抗原(SLA/LP)抗体、抗肝肾微粒体抗体I型(LKM-1)、抗肝细胞浆抗体1型(LC-1)、抗线粒体抗体Ⅱ型(AMA-M2),并分析各组阳性率。
补充资料：精子间接免疫荧光法
精子间接免疫荧光法
诊法。免疫学检查方法之 一。用于不育症检查。①以志愿供者的精液02ml加入10～12ml生理盐水中离心，取其精子沉淀部分再置入05ml生理盐水内。②取一滴作玻璃涂片，并在空气内晾干30分钟，用纯甲醇固定30分钟，再晾干后置入pH为74的磷酸缓冲液内15分钟。③被测血清滴于上述制备的玻片上，室温下孵化30～60分钟后，用磷酸缓冲液漂洗3次，每次10分钟。④玻片上滴注 附有抗人体免疫球蛋白抗血清的异硫氰酸荧光素盐酸盐，室温下孵化30～60分钟，孵化后用磷酸缓冲液漂洗3次，每次10分钟。⑤荧光显微镜下观察结果。利用血清内的精子抗体，可使精子不同部位呈现不同免疫荧光素反应，通过与正常血清对比研究，以判断精子抗体的存在情况。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条