Construction and screening of genomic library from Raji cells;

Construction of a bacterial artificial chromosome (BAC) genomic library of Heilongjiang River carps (C. carpio haematopterus)

DNA of GXP04 was digested with EcoRⅠ,and ligated with cosmid vector pLAFR3 to construct a genomic library.

Using the pSVhygB the genomic DNA library of Magnaporthe grisea was constructed.

Results:A genomic DNA library was constructed which included 11520 clones with average insert sizes about 1.

There are many ways to achieve the purpose,such as saving living bodies,establishing stem cell bank,mutant bank,generative cell bank,cDNA library,genome library and so on.

The rat genome library which has PBR322 as vector and 4～10 kb DNA segments cut by BamHI restriction enzyme as aim segments has been successfully constructed and identified.

In order to study the structures and functions of APP surface proteins,the genome library was constructed.

AIM:To construct a cDNA subtractive library of differential response genes of human fetal hepatocytes after HBV infection with suppression subtractive hybridization technique, and then to clone and identify the new genes that only express in HBV infected hepatocytes.

Then a subtractive library of the implantation site was successfully constructed by suppression subtractive hybridization (SSH).

Methylated genomic fragments were enriched by 3 cycles of NotⅠgenomic DNA subtractive hybridization and were linked to vectors,which was then used for transformation,fol.