1) random ized terminal linker-dependent PCR
依赖随机化末端连接物聚合酶链反应
2) Randomized terminal linker-dependent PCR
依赖随机化末端连接物聚合酶链式反应
3) RDPCR
依赖随机化末端连接物PCR
1.
Then the genomic DNA was amplified by randomized terminal linker-dependent PCR (RDPCR), and hybridized by Southern hybridization with the single-stranded probes of the exon 2 of k-ras gene.
酶切基因组DNA构建损伤模型,应用依赖随机化末端连接物PCR(RDPCR)进行Southern blot,对产物进行测序。
2.
Methods The TK6 cell was treated with BaP(benzo[a] pyrene),then its genomic DNA was amplified by RDPCR and detected by southern blot with the single-stranded probes of the exon 2 of k-ras gene.
方法苯并a芘(BaP)染毒细胞提取基因DNA,采用依赖随机化末端连接物PCR(RCPCR)进行核酸杂交及产物测序。
4) Arbitrarily primed polymerase chain reaction
随机引物聚合酶链反应
1.
Arbitrarily primed polymerase chain reaction for genotyping of meticillin resistant Staphylococcus aureus strains;
随机引物聚合酶链反应对耐甲氧西林金黄色葡萄球菌基因分型的研究
5) Random polymerase chain reaction
随机聚合酶链反应
6) ligation mediated polymerase chain reaction
连接介导聚合酶链反应
补充资料:聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条