1) differential centrifugation

差速离心法
1.
It could also be extracted from infected chicken embryo fibroblast (CEF) with differential centrifugation.
用免疫沉淀法从感染鸡法氏囊组织中分离传染性法氏囊病病毒(IBDV),用差速离心法从感染的鸡胚成纤维细胞(CEF)中分离IBDV,再从分离的IBDV中抽提dsRNA。
2) hypothermal differential centrifugation

低温差速离心法
3) differential centrifugation

差速离心
1.
Objective: To compare the efficiency between the traditional concentrated method (differential centrifugation) and simple and convenient method of the recombinant retrovirus.
目的比较传统逆转录病毒浓缩方法(差速离心)与简便方法浓缩逆转录病毒效率的差异。
2.
A method was developed for purification of 20S proteasome(20S core particle,CP) by combining differential centrifugations with nondenaturing polyacrylamide gel electrophoresis(native-PAGE),irrespective of species origins of CPs.
通过整合差速离心和非变性聚丙烯酰胺凝胶电泳(native-PAGE)技术,建立了能有效分离20S蛋白酶体(20S core particle,CP)的方法。
3.
Both total proteome and differential proteins were effectively extracted,separated and selected by a combined approach of both differential centrifugation and 2D-PAGE in liver of Paralichtys olivaceus(POL)under the stress of cadmium chloride as an artificial pollution source.
人工构建镉盐污染源,选用差速离心结合双向凝胶电泳(2D-PAGE)法,高效提取、分离和筛选牙鲆(Paralichthys olivaceus,PO)受镉盐胁迫后的肝脏全蛋白和差异蛋白质。
4) high-speed centrifugation

高速离心法
1.
29 - 1996"The Determination of Benzoic Acid and Sorbic Acid in Food",the practical feasibility of high-speed centrifugation in the purification of samples was investigated through a lot of analyses and comparisons.
29-1996食品中苯甲酸和山梨酸的测定方法)为基础,通过大量分析对比高速离心法对样品净化在实践中应用的可行性。
5) Ultracentrifugation

超速离心法
1.
Methods By using TCA/Acetone-precipitation,ultracentrifugation and modified ultracentrifugation,rat cortex samples were prepared and the effects of different protein extraction methods on the electrophoresis results were compared.
方法分别应用三氯乙酸/丙酮沉淀法、超速离心法及改良超速离心法制备电泳样品,并比较各处理方法对电泳的影响。
2.
METHODS: Size exclusion chromatography and ultracentrifugation were used to isolate and ascertain the G4 and Gl molecular forms of AChE.
通过超速离心法确证其沉降系数为10S和4S,分别为乙酰胆碱酯酸G4和G1分子亚型。
3.
METHODS The SLN and free drugs were separated by sephadex filtration,ultracentrifugation and mini-column centrifugation.
方法分别采用凝胶柱过滤法、超速离心法、微柱离心法分离固体脂质纳米粒和游离药物,并进行方法学考察,优化包封率测定条件。
6) low-speed centrifugation

低速离心法
1.
Determination of entrapment efficiency for fluorescence red GG liposomes by low-speed centrifugation
低速离心法测定荧光红GG脂质体包封率
补充资料:差速离心
分子式:
CAS号:
性质:生物技术中一种离心分离颗粒性物质的方法。采用逐渐增加离心速度或低速和高速交替进行离心,使沉降速度不同的颗粒,在不同离心速度和不同离心时间下分批分离。优点是技术简单,分离速度快,可以使用容量较大的角式转子;缺点是分离效果差,壁效应严重,颗粒被挤压。一般用于分离沉降系数相差较大的颗粒,如细胞器和病毒等。
CAS号:
性质:生物技术中一种离心分离颗粒性物质的方法。采用逐渐增加离心速度或低速和高速交替进行离心,使沉降速度不同的颗粒,在不同离心速度和不同离心时间下分批分离。优点是技术简单,分离速度快,可以使用容量较大的角式转子;缺点是分离效果差,壁效应严重,颗粒被挤压。一般用于分离沉降系数相差较大的颗粒,如细胞器和病毒等。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条