Inhibition of goose paramyxovirus(GPMV) replication by RNA interference targeted to matrix protein NP and L gene in CEF

According to nucleotide sequence of goose paramyxovirus(GPMV)NA-1 strain,two pairs of primers were designed and synthesized.

A new viral disease caused by goose paramyxovirus is an acute and violent infectious disease for geese, which characterized high mortality and result to a serious disserve in goose breeding industry.

Goose-host paramyxovirus strain NA-1 cDNA 5′-terminal end were determined by cRACE;The primers were designed described by Peter etc to determine the goose-host paramyxovirus strain NA-1 cDNA 3′-terminal end.

Establishment and application of digoxigenin-labeled nucleic acid probe for GPMV;

Pathogenic Analysis of NDV and GPMV to Embryo Fibroblasts;

In this study , We establish a rapid and sensitive sandwich immunochromatographic strip(ICS) for goose paramyxovirus (GPMV) detection.

Development of an indirect-ELISA assay based on NP protein of goose paramyxovirus and detection of antibody levels in immunized geese;

Cloning and sequencing of HN gene of goose paramyxovirus isolated from Liaoning;

m with the largely extracted pVAXⅠ-HN plasmid and empty vector pVAXⅠ to observe the immune effect of eukaryotic expression vector of goose paramyxovirus(GMPV) HN gene in mice,and the T lymphocyte proliferation activity and serum antibody against GPMV were detected by MTT and indirect ELISA method.

Analysis on antigenic variation between goose paramyxovirus strain NA-1 and F48E9;

Goose paramyxovirus disease with a high morbidity and mortality,is caused by Goose paramyxovirus(GPMV),a member of Paramyxoviridae.