1) Rapid amplification of cDNA ends
RACE
1.
The porcine CD8α full-length cDNA was amplified and cloned from total RNA of lymphocytes by RT-PCR and RACE(rapid amplification of cDNA ends).
应用RT-PCR和RACE技术从猪胸腺细胞总RNA中扩增克隆了猪CD8α的全长cDNA基因。
2.
By the methods of RT-PCR and rapid amplification of cDNA ends(RACE) we isolated a HSP70 gene(CsHSP70) from Capparis spinosa,a specific resistant plant in arid zone.
以干旱区特有的抗逆性植物刺山柑为材料,利用RT-PCR结合cDNA末端快速扩增(rapid amplification ofcDNA ends,RACE)方法,克隆了一个HSP70基因,将该基因进行原核表达,通过对大肠杆菌进行温度胁迫实验,并计算存活率来验证该基因对细胞的保护作用。
2) 3 RACE
3'RACE
1.
3 RACE Amplification and Sequence Analysis of a Strain of Norovirus;
一株诺如病毒的3’RACE扩增及基因序列分析
2.
Objective:To amplify the 3 -cDNA end of phospholipase D(PLD)gene of Agropyron mongolicum Keng by 3 RACE technique and to analyze the sequence in the experiment,the 3 -cDNA end cloning establish foundation in order to obtain the cDNA sequence and research the function of PLD gene.
目的:采用3’RACE方法对蒙古冰草磷脂酶D(PLD)基因3’端全序列进行了快速扩增和序列分析,为得到全序列及研究该基因的功能奠定基础。
3.
The cDNA of DMrt1(DM-related transcription factor1)was amplified by 3 RACE-PCR(Rapid Amplification of cDNA Ends-Polymerase chain reaction)strategy using the total isolated RNA as template.
从奥利亚罗非鱼精巢提取总RNA,采用RT和3’RACE(rapid amplification of cDNA ends)法分离和测定了奥利亚罗非鱼DMRT1(DM-related transcription factor1)cDNA的3’序列,扩增得到1086bp的片段,阅读框共编码254个氨基酸,序列分析结果表明,奥利亚罗非鱼DMRT1基因的氨基酸序列与尼罗罗非鱼DMRT1基因的同源性为99%,与黑鲷、黄鳝、新月鱼、虹鳟、青鳉鱼等动物的DMRT1cDNA和尼罗罗非鱼、奥利亚罗非鱼等动物的DMO(DM-domain gene in Ovary)cDNA的同源性分别为:80%、78%、73%、62%、41%、26%、26%。
3) 3'-RACE
3-'RACE
1.
In this study,a cDNA fragment of dehydroascorbate reductase(DHAR) including whole 3 -end was cloned from Royal Gala by RT-(nest) PCR and 3′-RACE.
以皇家嘎拉苹果叶片为材料提取总RNA,合成cDNA第一链后,先利用巢式PCR获得苹果脱氢抗坏血酸还原酶(DHAR)基因中间片段,再利用3-′RACE方法进一步获得3′末端核酸序列,共得到840 bp包含完整3′末端的DHAR基因cDNA片段,其序列与烟草、水稻、番茄中DHAR基因序列的同源性均大于70%,证明所克隆到的核苷酸片段确为苹果DHAR基因cDNA片段。
4) 3-RACE
3'-RACE
1.
Amplification Carbo-tip of L Gene by 3 -RACE and Sequence Analysis of Rabies Virus;
RV野毒株L基因C端3’-RACE扩增及序列分析
2.
Methods:An objective gene was isolated by using a RT-PCR and 3 -RACE approachs and two degenerate PCR primers against conserved sequences in the MADS-box and in the C-terminal region of several plants.
方法:根据多种植物的MADS-box保守区及其C-端序列设计一对简并性引物,利用RT-PCR及3’-RACE的方法分离目的基因。
6) 3 RACE or 5 RACE
3 RACE或5 RACE
补充资料：2-isopropyl-5-methyl-cyclohexan-1-on racemic
CAS: 1074-95-9
分子式: C10H18O
中文名称: 反-DL-5-甲基-2-(1-甲基乙基)环己酮
英文名称: 5-methyl-2-(1-methylethyl)-, trans-(+-)-Cyclohexanone；dl-p-menthan-3-on；2-isopropyl-5-methyl-cyclohexan-1-on racemic；trans-(+/-)-cyclohexanon；menthone racemic；p-menthan-3-one racemic
分子式: C10H18O
中文名称: 反-DL-5-甲基-2-(1-甲基乙基)环己酮
英文名称: 5-methyl-2-(1-methylethyl)-, trans-(+-)-Cyclohexanone；dl-p-menthan-3-on；2-isopropyl-5-methyl-cyclohexan-1-on racemic；trans-(+/-)-cyclohexanon；menthone racemic；p-menthan-3-one racemic
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条