In order to know the regularities of zygotic embryo development of Pinus yunnanensis,the green cones were collected from Kunming Arboretum throughout the summer of 2006～2007.

It results in the abortion of the zygotic embryo, due to the competition with the nucellar embryos, which are often more vigorous.

13, small heat-shock protein seems, was to be thought to relate to the zygotic embryo development processing, respectively.

With the mature zygotic embryos of antidisease families and non-antidisease families of slash pine as explants,the key technique and main factors affecting vitro culture multiplication of slash pine were studied,which were based on different cultures,hormone compositions in different concentration ratio as well as different plant growth regulators.

We researched the affecting factors of induction and proliferation on Pinus koraiensis callus by using mature and immature zygotic embryos as explants and disscussed the induction factors of shoot regeneration.

Rapid adventitious bud induction of Betula platyphylla was establinshed from mature zygotic embryos.

In cytological study of embryo development, by the way of paraffin, somatic and zygotic embryogenesis was observed and compared under light microscope.

Using mature zygotic embryos as initial explants, the effect factors including basal medium and plant hormone on organogenesis, somatic embryogenesis and adventitious buds induction roots were investigated respectively.

In this paper,the materials was the mature zygotic embryos of Picea mongolica, induced embryogenic callus and subculture, which established a good dispersion and a high dynamic suspension cell line.