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1)  Transgenic rice of PEPC
转玉米PEPC基因水稻
2)  PEPC transgenic rice
转PEPC基因水稻
1.
Characteristics of tolerance to photoinhibition and photooxidation characteristics in PEPC transgenic rice;
转PEPC基因水稻耐光抑制和光氧化的特性
2.
The eighth generation of PEPC transgenic rice and an untransformed rice cultivar(Oryza sativa L.
本文运用稳定和放射性碳同位素研究了第8代转PEPC基因水稻,并测定其光合速率和荧光特性。
3.
The photosynthetic properties, yield character of PEPC transgenic rice inoculated nitrogen-fixing bacteria and nitrogen-fixing capacity of nitrogen-fixing bacteria inoculated in PEPC transgenic rice were studied by using l5 N-isotope tracer technique.
利用~(15)N-同位素技术研究转PEPC基因水稻及无PEPC基因水稻在接种不同固氮菌后的光合特性、产量性状和固氮能力的变化。
3)  maize pepc gene
玉米pepc基因
1.
By cross breeding,the maize pepc gene in the pepc transgenic rice was successfully incorporated into the parents of two-,three-line hybrid rice,including sterile lines (Peiai64S,2302S,2304S,2306S and Shuangjiu A) and restorer lines (5129,02428 and Wanjing97) to breed the high-photosynthetic efficiency parents of hybrid rice and utilize heterosis between C_4 and C_4/C_3 rice.
在合肥、海南两地以高光效转玉米pepc基因水稻为父本 ,与培矮 64S、2 3 0 2S、2 3 0 4S、2 3 0 6S、512 9、0 2 42 8、皖粳97和双九A等 8个受体杂交 ,转育转pepc基因水稻新种质材料。
4)  transgenic rice with pepc gene
转pepc基因高光合水稻
5)  transgenic rice
转基因稻米
1.
This study deals with the polymerase chain reaction (PCR) detection technology of the marker gene bar , CaMV 35 S promoter and Nos terminator DNA sequence in both crude transgenic rice and its processed food stuffs.
本文对转基因稻米及其加工食品进行了针对标记基因bar、CaMV35S启动子和Nos终止子DNA 序列的PCR检测。
2.
The authors of the present paper compared the seed protein fingerprints of the transgenic rice and their rotation parents,and identified their equivalence at the genetic and biochemical level.
结果表明 ,通过常规杂交转育手段将转基因导入水稻品种后 ,经过选育转基因稻米的可溶性蛋白质组分可与轮回亲本达成一致 ,仅个别组分的含量发生改变。
6)  Genetically modified maize
转基因玉米
1.
The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.
通过调整非同源模板浓度使竞争物PCR产物亮度与1%GMO玉米的亮度相同,从而确定转基因玉米的检出限为1%。
2.
According to the plasmid map of Genetically Modified Maize,we select exogenous genes(CaMV35S promoter,NOS terminator,PAT gene,IVS2/PAT,CDPK/CryIA(b),Maize genome/CaMV35S,PAT/CaMV35S!,Cry9C/CaMV35S!) and endogenous gene(IVR) as target genes,design and synthesize their primers.
根据转基因玉米中所转入的外源基因,选择CaMV35S启动子、NOS终止子、PAT基因和目的基因IVS2/PAT、CDPK/CryIA(b)、Maize genome/CaMV35S、PAT/CaMV35S!、Cry9C/CaMV35S!以及内源IVR基因设计特异性引物,采用多重PCR法对待测样品进行扩增,通过缺口平移法合成DIG-dUTP标记杂交探针,并制备基因芯片。
补充资料:水稻基因组物理图谱
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性质:中国于1992年8月正式宣布实施《水稻基因组计划》,并在上海成立了中国科学院国家基因研究中心。于1996年6月完成了水稻基因组物理图的构建。该基因组由十二条染色体组成,其DNA总长为4.3亿核苷酸。简单地说,物理图指的是将水稻基因组DNA用DNA限制性内切酶切割成20 000多DNA片段,再按其基因组天然的结构将这些片段重新排列成十二条染色体。已完成的水稻基因物理图中的DNA片段平均长度为120Kb。该物理图有两大用途。第一,在物理图上把所需的基因限定在一定的范围内,结合定位克隆(mapbased cloning)技术可获得所需基因,对农业育种和理论研究十分有利。第二,在完成对每一DNA片段测序的基础上,可使人类在核苷酸水平上全面解开水稻的遗传之谜。

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