To generate a loxPneoloxP selection cassette for construction of goat mammary gland gene targeting vectors, loxP sites were introduced into both the forward and reverse primers and a plasmid pTarget containing neo gene was used as the template for high fidelity PCR amplification.

The considerable exploit of lactic acid bacteria and the development of molecular biology have allowed to construct many non-antibiotic resistance selection markers for LAB that can be categorized into four kinds: dominant selection marker, complementation sel.

Study of selectable markers in genetic engineering of Chlorella sp.;

A transformation vector with the mutated Bm1 gene from Neurospora crassa as the selectable marker to benomyl and under the transcriptional control of the gpd promoter and terminator from the rice blast fungus,Magnaporthe grisea(isolate 0742),was used to transform M.

Some transformation methods,selective markers,and the application of transformation system in the field of research on filamentous fungi are reviewed in this paper.

Recent achievement in the field of genetic engineering of edible mushroom, including genomics, gene isolation and coloning, selective markers and methods in genetic transformation were reviewed in this pape

It suggests that CAT gene could be a suitable selective marker for D.

Construction of the a-ACO1 gene of sweet melon expression vector with marker-free and its co-transformation to tobacco;

Research on marker-free transformation of Brassica napus with Fad2 antisense gene and RNA interference;

Research on Transformation of Maize with Marker-free Expression Vector of BADH and CMO Genes;