1) Hatched mouse blastocyst

小鼠孵化囊胚
1.
Hatched mouse blastocysts were vitrified in liquid nitrogen after equilibration in 10% or 20% GL for 5min and in GFS40 for 30sec respectively, the survival rates were 93%- 97% after the frozen-thawed embryos were cultured in vitro for 24h.
5min二步法冷冻保存的小鼠孵化囊胚,解冻后24h内恢复率高达93%—97%,与对照组(99%)相比无显著差异(P>0。
2) hatched blastocyst

孵化囊胚
1.
Vitrification of mouse hatched blastocysts by straw and OPS mehtod;

小鼠孵化囊胚细管法和OPS法玻璃化冷冻保存技术的研究
2.
Hatched blastocysts were plunged into liquid nitrogen after short exposure to EFS40 at 25℃ (one- step method), or the embryos were vitrified in EFS40 after pretreatment in 10% EG or 20% EG(two- step method).
采用两种方法对家兔孵化囊胚作玻璃化冷冻保存:在 25℃室温下,将孵化囊胚直接移入 EFS40溶液中短时间平衡后,直接投入液氮中冷冻保存 (简称一步法 );或将胚胎在 10%或 20% EG溶液中经预处理后,再移入 EFS40溶液冷冻保存 (简称二步法 )。
3) hatching rate of blastula

囊胚孵化率
4) mouse blastocysts

小鼠囊胚
1.
Vitrification of mouse blastocysts using two kinds of straw systems;

应用两种麦管系统对小鼠囊胚的玻璃化冷冻
5) ICR mice blastocysts

ICR小鼠囊胚
1.
Methods: ES cells were isolated from C57BL/6J mice blastocyst inner cell mass, and cultured for 15 passages, then injected into blastococels of ICR mice blastocysts to create chimeric mice.
经显微注入ICR小鼠囊胚腔中,产生4只嵌合体小鼠,经证实其中1只为种系嵌合小鼠。
6) mouse tooth germ

小鼠牙胚
1.
RESULTS:Positive staining for USF1 mRNA in mouse tooth germ began from P1d evidently,and extended to P11d,which was mainly localized in the secreting ameloblasts and odontoblasts,but no staining in b.
结果:原位杂交染色从钟状晚期小鼠牙胚中开始检测到阳性信号,持续至P11d,阳性信号主要定位于成牙本质细胞与成釉细胞;而蕾状期,帽状期,钟状期牙胚中未检测到阳性信号,并且在牙齿萌出后(P21d),牙齿中USF1mRNA阳性信号再次消失。
补充资料:L(+)-鼠李糖一水合物 鼠李糖,一水
CAS:10030-85-0
分子式:C6H14O6
分子质量:182.17
熔点:91-93℃
中文名称:L(+)-鼠李糖一水合物 鼠李糖,一水
英文名称:L(+)-Rhamnose monohydrate; Rhamnose,monohydrate iso-Ducitol
用途:用作生化试剂。
分子式:C6H14O6
分子质量:182.17
熔点:91-93℃
中文名称:L(+)-鼠李糖一水合物 鼠李糖,一水
英文名称:L(+)-Rhamnose monohydrate; Rhamnose,monohydrate iso-Ducitol
用途:用作生化试剂。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条