1) transfection helpmate
转染伴侣
1.
Taking ad- vantage of this effect, a new gene transfection method utilizing SiMP as transfection helpmates was devel- oped.
报道了二氧化硅微颗粒(SiMPs)的促细胞增殖效应,并基于该效应发展了一种以二氧化硅微颗粒为转染伴侣的基因转染新方法,采用MTT实验证明了二氧化硅微颗粒具有促细胞增殖效应,并以绿色荧光蛋白表达载体质粒pEGFP为报告基因,COS-7细胞为靶细胞,在多聚-L-赖氨酸介导的基因转染中,利用二氧化硅微颗粒的促细胞增殖效应,加入二氧化硅微颗粒作为转染伴侣开展基因转染实验,获得了显著增强的基因转染效率,相比于未加入二氧化硅微颗粒为转染伴侣的基因转染方法,该方法的转染效率提高到5倍多。
2) copper chaperone
转铜伴侣
1.
Study on the copper-induced conformational changes of human copper chaperone Atox1;
铜诱导人体转铜伴侣蛋白Atox1构象变化的研究
2.
Function and structural properties of copper chaperones;
转铜伴侣的功能与结构特性
3.
cDNA of human copper chaperone Atox1 was amplified by PCR and cloned into PCRII vector After DNA sequencing, it was cloned into GST fusion protein expressive vector PGEX 6p 2.
利用PCR技术扩增出人体转铜伴侣蛋白Atox1的cDNA片段 ,直接克隆到PCRII载体上 ,经DNA序列测定后 ,再插入到谷胱甘肽巯基转移酶 (GST)融合表达载体PGEX 6p 2上 ,构成重组表达质粒PGEA ,将此质粒导入大肠杆菌 ,经IPTG诱导后获得PGEA融合蛋白的表达 表达的融合蛋白经亲和层析、位点特异性蛋白酶切得到纯化的Atox1蛋
3) companion
[英][kəm'pæniən] [美][kəm'pænjən]
n.同伴，伴侣
4) artificial chaperone
人工伴侣
1.
Cooperation of osmolytes and artificial chaperone on malate dehydrogenase refolding;
渗透剂与人工伴侣耦合辅助苹果酸脱氢酶包含体的复性
2.
The effect of guanidine hydrochloride (GuHCl) concentration on the oxidative refolding of denatured-reduced lysozyme of high concentration in the presence of artificial chaperone, that is, cetyl trimethyl ammonium bromide (CTAB) and β -cyclodextrin ( β -CD),was studied.
研究了表面活性剂十六烷基三甲基溴化铵 (CTAB)加 β -环糊精 (β -CD) (人工伴侣系统 )和盐酸胍对高浓度变性 -还原溶菌酶氧化复性的影响 ,利用基于二态复性模型的表观动力学模型分析了溶菌酶的复性过程 。
3.
A kinetic model based on the competition between protein folding and aggregation was employed to express the refolding process of denatured/reduced lysozyme by direct dilution and that facilitated by the artificial chaperone system (ACS), i.
利用复性和聚集竞争反应动力学模型描述自发复性和人工伴侣系统(十六烷基三甲基溴化铵与b -环糊精)促进溶菌酶复性动力学。
5) artificial chaperones
人工伴侣
1.
Renaturation of high concentration lysozyme using gel filtration chromatography assisted by artificial chaperones;
人工伴侣与凝胶过滤色谱耦合作用促进高浓度溶菌酶复性研究
2.
In order to explore the best concentration of glutathione in the test assisting with artificial chaperones by one-step method,the hydrochloric acid-Denatured and DTT-Reduced Lysozyme are taken as the model protein.
利用盐酸胍变性、二硫苏糖醇还原的溶菌酶为模型蛋白,考察人工伴侣一步法辅助溶菌酶复性时的最佳谷胱甘肽浓度。
3.
Based on the improvement of measuring the standard curve of lysozyme activity, we optimized the conditions of the lysozyme refolding assisted by artificial chaperones.
本文在改进溶菌酶标准曲线测定方法的基础上,优化了人工伴侣辅助溶菌酶复性的各种条件,进一步研究了人工伴侣与小分子添加剂协同辅助高浓度溶菌酶的复性。
6) molecular chaperone
分子伴侣
1.
Effect of ATP on molecular chaperone function and conformation of α-crystallin;
ATP对α-晶状体蛋白分子伴侣功能及构象的影响
2.
Change of molecular chaperone activity of high-molecular-weight alpha-crystallin in age-related cataract;
高相对分子质量α-晶体蛋白分子伴侣活性在年龄相关性白内障中的变化
3.
Effects of fructose 6-phosphate and ribose on molecular chaperone activity of α-crystallin;
6-磷酸果糖和核糖对α-晶状体蛋白分子伴侣活性的作用
补充资料：世间转法转智转无尽
【世间转法转智转无尽】
世间转者，谓展转摄前众生界、世界、虚空界也。法转者，谓展转摄前法界、涅槃界、佛出现界也。智转者，谓展转摄前如来智界，心所缘佛智所入境界也。盖此三转，皆言无尽者，以世法智之三种，展转含摄，无有穷尽，是名世间转、法转、智转无尽。
世间转者，谓展转摄前众生界、世界、虚空界也。法转者，谓展转摄前法界、涅槃界、佛出现界也。智转者，谓展转摄前如来智界，心所缘佛智所入境界也。盖此三转，皆言无尽者，以世法智之三种，展转含摄，无有穷尽，是名世间转、法转、智转无尽。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条