1) Pichia pastoris
巴斯德毕氏酵母
1.
Secreted Expression of Mature Peptide Gene of Human Bone Morphogenic Protein-7 in Pichia pastoris;
人骨形成蛋白-7成熟肽基因在巴斯德毕氏酵母中的分泌表达
2) Pichia pastoris
巴斯德毕赤氏酵母
1.
Cloning and Expressing of gsh 1 gene in Pichia pastoris;
gsh 1基因的克隆及其在巴斯德毕赤氏酵母中的表达
2.
Expression of Monellin, a Sweet Protein, in Pichia pastoris;
甜蛋白Monellin在巴斯德毕赤氏酵母中的胞内表达
3.
Objective:Construction of one novel recombinant Pichia pastoris for production of glutathione,and to study its glutathione synthesis capacity.
目的:构建一株新型的产谷胱甘肽的重组巴斯德毕赤氏酵母,并研究该重组菌合成谷胱甘肽的能力;方法:利用PCR技术克隆来源于酿酒酵母的γ-L-谷氨酰-L-半胱氨酸合成酶编码基因GSH1和谷胱甘肽合成酶的基因GSH2,串联插入表达载体pGAPZA,转化Pichia pastoris GS115,在Zeo-cin平板上挑选阳性转化子,并对阳性转化重组菌进行摇瓶培养筛选。
3) Pichia pastoris
巴斯德毕赤酵母
1.
Effect of Ammonium Concentration on the Expression of Angiostatin in High Cell Density Culture of Recombinant Pichia pastoris;
重组巴斯德毕赤酵母高密度培养中铵离子浓度的影响
2.
Optmization Expression of Saliva Histain 5 Mutant in Pichia Pastoris;
唾液Histatin5突变体在巴斯德毕赤酵母中的优化表达
3.
Expression of recombinant human A20 in Pichia pastoris GS115;
重组人A20蛋白在巴斯德毕赤酵母GS115中的表达
4) Pichia pastoris
毕赤巴斯德酵母
1.
The phytase phyA gene recombinant plasmid pPIC9K-PhyA was introduced into Pichia pastoris GS115 strain by electroporation after being linearnized with Bglll.
本研究以本课题组构建的植酸酶phyA基因表达载体pPIC9K-PhyA为材料，采用电击转化法将phyA基因导入毕赤巴斯德酵母(Pichia pastoris)。
2.
Secretory expression of Bacillus subtilis phytase phyC in Pichia pastoris;
根据枯草芽孢杆菌WHNB02植酸酶phyC基因全序列设计一对引物,采用PCR法从含有该基因的pUC18-phyC质粒上获得不含有信号肽序列的phyC基因表达片段,亚克隆到毕赤巴斯德表达载体pPIC9K的多克隆位点,并电转化毕赤巴斯德酵母GS115。
5) engineering P.pastoris
毕赤氏巴斯德酵母工程菌
6) Pichia pastoris
巴斯德毕赤酵母菌
1.
High-level expression and secretion of recombinant human Sjogren s syndrome antigen B in methylotrophic yeast Pichia Pastoris;
人干燥综合征B抗原在巴斯德毕赤酵母菌中的高效分泌表达研究
2.
AIM: To find if human soluble tumor necrosis factor receptor II (p75) fused IgG Fc protein (sTNFR II-IgG Fc) could be expressed in Pichia pastoris with an active dimmer form and characterize its N-linked oligosaccharides.
以重组载体转化巴斯德毕赤酵母菌中进行克隆与表达。
补充资料：巴德氏征