1) promoter-pGL3-Basic-GFP expressed vector
pGL3-Basic-GFP表达载体
2) pGL3-basic plasmid
pGL3-basic真核表达载体
1.
Methods Different lengths CDA1P were amplified by PCR using mouse liver genomic as template and cloned into BglⅡ digested pGL3-basic plasmid to produce recombinant plasmid pGL3-basicmCDA1P,then they were transfected into Lewis lung cell line(LLC) and RAW 264.
方法:以小鼠肝脏的全基因序列为模板,用PCR方法获得不同长度的目的片段,连接到pGL3-basic真核表达载体(pGL3-basic-mCDA1P),纯化pGL3-basic mCDA1P质粒后,瞬时转染到Leuwis肺癌细胞和单核巨噬细胞(RAW264。
3) pGL3-Basic vector
pGL3-Basic载体
1.
Then P4 promoter gene fragment was inserted into the KpnI-HindⅢsite of pGL3-Basic vector,to construct pGL3-Basic eukaryotic expression vector bearing P4 promoter gene,pGL3-P4,which was further confirmed by double enzyme digestion and DNA sequencing.
成功克隆IGF-ⅡP4启动子基因,并构建P4启动子的pGL3-Basic载体。
2.
After three promoter segments with different lengths were amplified by PCR amplification,and then the products were identified by DNA sequencing,four promotor segments were inserted into pGL3-Basic vectors.
方法运用在线软件Cister对CCDC72基因5’端2000bp进行启动子特征分析;以人毛乳头细胞基因组DNA为模板进行PCR扩增目并进行测序鉴定,再以扩增产物为模板PCR得到不同长度的启动子片段,并测序,将获得启动子片段插入pGL3-Basic载体。
6) GFP fusion expression vector
GFP融合表达载体
补充资料：Sulfuric acid, copper salt, basic、copper sulfate(basic)
CAS：1344-73-6
中文名称：碱性硫酸铜盐
英文名称：Sulfuric acid, copper salt, basic、copper sulfate(basic)；coppersulfate(basic)；Sulfuric acid,copper salt,basic
中文名称：碱性硫酸铜盐
英文名称：Sulfuric acid, copper salt, basic、copper sulfate(basic)；coppersulfate(basic)；Sulfuric acid,copper salt,basic
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条