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1)  immunoaffinity column chromatography
免疫亲和柱层析
1.
The manifold antibodys with different chromatography behaviors were discovered through immunoaffinity column chromatography or Fe 3+ metal chelated.
免疫亲和柱层析或金属(Fe3+ )螯合亲和柱层析后,白蛋白抗体样品均出现多个不同层析行为的抗体峰。
2)  immunoaffinity chromatography
免疫亲和层析
1.
A novel strategy for subtracting high-abundance proteins in human milk by immunoaffinity chromatography;
利用免疫亲和层析技术去除母乳中高丰度蛋白的研究
2.
Study on purification of rhEPO by the immunoaffinity chromatography;
免疫亲和层析法纯化重组人促红细胞生成素的实验研究
3.
The method for trace analysis of microcystin-LR in water using immunoaffinity chromatography column and high performance liquid chromatograph was established,its limit of detec.
用CNBr-activated Sepharose4B和微囊藻毒素-LR的单克隆抗体制备了免疫亲和层析柱,测得抗体偶联率在75。
3)  Immuno-affinity chromatography
免疫亲和层析
1.
Membrane protein of Mycoplasma hyopneumoniae from ATCC25095 strain was extracted and purified by immuno-affinity chromatography.
通过提取猪肺炎霉形体ATCC25095株膜蛋白,并用免疫亲和层析柱对膜蛋白进行纯化,经SDS-PAGE分析得到了分子质量分别为66、50、46、42、32和24 ku的6种膜蛋白。
4)  immunoaffinity column
免疫亲和柱
1.
Rapid Determination of Aflatoxin B_1,B_2,G_1,G_2 in wine with HPLC and Immunoaffinity Column;
免疫亲和柱HPLC荧光检测酒中黄曲霉毒素B_1、B_2、G_1、G_2
2.
A method has been developed for the determination of T-2 toxin in cereal grains by high performance liquid chromatography with fluorescence detection after immunoaffinity column clean-up and precolumn derivatization.
建立了免疫亲和柱净化/柱前衍生化-高效液相色谱荧光检测器测定粮谷中T-2毒素含量的方法。
3.
Rapid screen determination method (fluorometry method) and rapid quantitation method (high performance liquid chromatography of ochratoxin A with immunoaffinity column cleanup) for spices and dried vine fruits have been developed.
针对香辛料和藤蔓水果干中可能污染的赭曲霉毒素A,采用免疫亲和柱层析净化手段,建立了快速筛选检测方法(荧光光度法)和快速确证方法(高效液相色谱法)。
5)  affinity cartridges
免疫亲和柱
1.
Determination of Fumonisins B_1,B_2 in Corn by Affinity Cartridges-High Performance Liquid Chromatography;
单克隆免疫亲和柱-高效液相色谱法测定玉米中伏马毒素B_1、B_2
2.
Filtrate is cleaned up by ZearalaTest affinity cartridges.
研究了单克隆免疫亲和柱 高效液相色谱法测定玉米中玉米赤霉烯酮的方法。
3.
The sample is cleaned up by FumoniTest affinity cartridges.
研究了单克隆免疫亲和柱 -高效液相色谱法测定酱油中伏马毒素B1、B2 的方法。
6)  immunoaffinity chromatography with magnetic bead
磁珠免疫亲和层析
1.
Purification of inhibin from porcine follicular fluid by immunoaffinity chromatography with magnetic beads;
应用磁珠免疫亲和层析法从猪卵泡液中提纯抑制素
补充资料:亲和层析
分子式:
CAS号:

性质:又称亲和色谱。利用生物大分子所具有的专一亲和力而设计的一种层析技术。用连接在基体的配位体(如酶、抗体、激素等)作为固定相,使与蛋白质或其他大分子发生可逆的高选择性的相互作用,利用不同亲和力进行分离的液相色谱法。(1)酶-底物或底物类似物或抑制剂或辅因子(辅酶、金属离子等);(2)抗体-抗原或病毒或细胞;(3)激素、维生素-受体蛋白或载体蛋白;(4)外源凝集素-多糖化合物或糖蛋白或细胞表面受体蛋白或细胞;(5)核酸-互被碱基链段或组蛋白或核酸聚合酶或核酸结合蛋白。层析柱的制备是将配体共价偶联到一个不溶性的基物上。样品溶液通过此层析柱时,与此配体能特异结合的组分便被吸附而与其他组分分开。然后,在不能发生特异结合的条件下用某些试剂淋洗以将该物质与配体重新拆开,这样便可获得纯化后的该组分。亲和层析法具有简便、快速、高效且分离产物不会失活等优点。其主要用途是分离和提纯蛋白质、膜和多糖类物质。

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