1) in vitro refolding
体外重折叠
1.
The differences in the in vitro refolding, oxidation of free thiol groups, circular dichroism (CD) spectra, antibody and receptor binding assays, and sensitivity to tryptic digestion between the mutant and the wild type proteins were studied.
为研究A7 B7二硫键在胰岛素原结构和折叠中的作用 ,构建了A7 B7二硫键缺失的胰岛素原突变体 ,研究了其与野生型胰岛素原在体外重折叠产率、自由巯基氧化速度、CD谱、受体及抗体结合活性 ,以及对胰蛋白酶酶切敏感性的差别。
3) protein folding in vitro
蛋白质体外折叠
4) outer folder
外折叠
1.
By experiment analysis including metallographic examination and SEM,researching on the sample from the outer folder defect location of the GCr15 tube,the decarburized depth and large-size non-metallic inclusions had been detected.
通过金相观察和扫描电镜的实验方法,对GCr15钢管外折叠缺陷处的微观组织以及脱碳层和非金属夹杂物的特性进行了检测分析,分析结果说明折叠根部脱碳层较严重并存在大尺寸B类Al_2O_3夹杂物等。
5) refolding
重折叠
1.
The refolding reaction of human group Ⅹ phospholipase A2 in vitro was depended on temperature, pH and protein concentration.
用以前适用于人胰型(IB 型) 磷脂酶A2 的稀释重折叠方法,并不能使它有效重折叠。
2.
After solubilization, active proteins can be generated through an appropriate refolding process.
包涵体富含表达的重组蛋白 ,经分离、变性溶解后须再经过一个合适的复性过程实现变性蛋白的重折叠 ,才能够得到生物活性蛋白。
3.
The unfolding and refolding of Bacillus amyloliquefaciensα-amylase induced with urea or guanidine hydrochloride were studied by using its intrinsic fluorescence emission spectroscopy,fluorescence phase diagram,fluorescence probe,fluorescence quenching, Native-PAGE,SDS-PAGE and Size exclusion chromatography.
本论文分别采用荧光发射光谱法、荧光相图法、荧光探针、荧光猝灭法,以及非变性聚丙烯酰胺凝胶电泳法、十二烷基磺酸钠-聚丙烯酰胺凝胶电泳法和体积排阻色谱法对尿素和盐酸胍诱导的淀粉液化芽孢杆菌α-淀粉酶的去折叠过程和重折叠过程进行了研究。
6) refolder
重折叠器
补充资料：外重
1.谓中央政权以外的地方藩镇势重。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条