METHODS: SP immunohistochemical staining and immunofluorescence were performed in paraformaldehyde fixed hippocampal sections.

METHODS Indirect immunofluorescence method and MTT method were used to test the expression of human IL-6 receptor on R2 cells and the reaction of R2 cells to recombinant human IL-6(rhIL-6).

Methods Immunohistochemistry was used to detect expression of ABCG2 in 40 cases of transitional cell carcinoma of bladder with different pathology grades(12 cases were Grade Ⅰ,18 cases were Grade Ⅱ,10 cases were Grade Ⅲ)and 15 normal bladder tissues,while immunofluorescence was used to detect expression of ABCG2 in BIU-87 cell line.

Expression of Survivin in leukemia by Western blot analysis and immunofluorescence method;

RuBPCase in leaf blade transection of typical C 3, C 4 plants and Hevea brasiliensis was located by direct immunofluorescence method.

Rabbit antiserum raised purified ribulose 1,5 bisphosphate carboxylase (RuBPCase) from tobacco was used to locate RuBPCase in leaf blade transection of classical C 3, C 4 plants and Coffea arabica by direct immunofluorescence method.

Establishment of the magnetic separation enzyme immunofluorescence method for detecting insulin;

The method to establish MGN model in mice and immunofluorescence quantitative analysis of this model;

The diagnosing value of endometrial immunoglobulin G antibodies with immunofluorescence assay in patients with endometriosis;

Immuno-fluorescence imaging of hepatocellular carcinoma cells with quantum dots;

Anti-IF-protein antibodies were used as probes for immuno-fluorescence,and the reactions of them with different parts of the cell were detected,which suggested the possibility of the existence of the intermediate-like filaments in the cytoplasm.