2) Feeder-free culture system
无饲养层培养
1.
Conclusion:Feeder-free culture system can be used on chinese human embryonic stem cell lines successfully and there are obvious differences between two chinese lines with others reported abroad in the double time,rate of differerentiated cells and density of feeder-layer.
2×106/mL)获得的条件培养基(CM)在无饲养层培养体系培养12代以上,用机械分离法和Ⅳ型胶原酶法进行传代,观察比较结果并对所得hESCs鉴定。
4) Un-odour breeding
无臭饲养
5) Feeder layer
饲养层
1.
Human dermal fibroblasts as a feeder layer promote the growth of human keratinocytes;
人成纤维细胞饲养层促进人角朊细胞生长
2.
Development of a G418-resistant feeder layer of embryonic stem cell of mice;
抗G418小鼠胚胎干细胞饲养层的制备
3.
Establishment and screening of feeder layer culture system for mouse embryonic stem cell;
小鼠胚胎干细胞饲养层培养体系的优化筛选
6) feeder layers
饲养层
1.
Mitomycin C was used to pretreat MEF for feeder layers of embryonic stem cell and MEF was digested for donor cells of nuclear transfer.
方法取昆明小鼠胚胎分离成纤维细胞,用丝裂霉素C处理成纤维细胞为胚胎干细胞制备饲养层,消化贴壁成纤维细胞为核移植提供供体细胞。
2.
Objective To perform the proteome analysis of conditioned medium prepared from mouse embryonic fibroblast feeder layers by 2-dimensional (2D) electrophoresis and mass spectrometry and to find out the possible differentiation-inhibitory factor in conditioned medium.
目的:运用双向电泳、质谱技术对小鼠胚胎成纤维细胞饲养层条件培养液进行蛋白质组学分析,寻找 其中可能具有抑制分化作用的蛋白因子。
3.
To overcome these problems, feeder layers originating from human fetal and adult tissues (including human fetal skin fibroblasts, fallopian-tube epithelial cells et al) have been tested.
饲养层(feeder cells)是体外成功培养人EG细胞的必要条件,因此寻找适合人EG细胞体外培养的饲养层是人EG细胞成功建系并用于实际的关键。
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