The propagation characteristics of virulent duck plague virus(DPV)in duck embryo fibroblast(DEF)were studied by the method of light microscopy observation of DEF cell culture monolayer,electron microscopy observation of infected DEF cell culture,real-time PCR detecting virus propagation.

Expression and Immunological Activity of E2 Epitope of Classical swine fever virus;

Pathogenicity of classical swine fever virus GX-3/98 strain and immunization relativity with attenuated hogcholera virus Chinese C strain;

Kinetics of peripheral blood leukocytes apoptosis and changes of CD4~+ and CD8~+ T lymphocyte subpopulations of pigs infected with classical swine fever virus;

The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting classical swine fever virus(CSFV) Shimen strain E2 gene into pBABE-puro.

A classical swine fever virus(CSFV) E2 gene was codon-optimized according to the Sf9 cell codon usage.

A truncated gene encoding the major antigenic domains of E2 protein of classical swine fever virus(CSFV) was amplified by RT-PCR from the genomic RNA of CSFV C-strain and cloned into pPROEX-HTb expression vector to obtain recombinant pPROEX-tE2.

Fusion expression of multiepitope gene of CSFV envelope glycoprotein E2 and its protection efficiency against homologous challenge in rabbits;

Prediction,synthesis and characteristics of B-cell epitopes of CSFV envelope glycoprotein E2;

Detection of the Interference to the Reproduction of CSFV by PK-15 Cell Strains Transcribing shRNA Targeted to the 3′-UTR;

Dynamics of IFN-α mRNA expression in liver of ducks infected with duck plague virus of different virulence;

Purification and electron microscopy observation after negative staining of duck plague virus;

Prokaryotic expression of N-terminal antigenic domain of duck plague virus gB protein and the establishment of putative indirect ELISA assay;