Then we will design and construct the eukaryotic expression vector of human Par-4 gene, transfect it into human leukemia cells K562, investigate the effect of Par-4 to WT1mRNA and protein expression level in K562, and finally observe the influence of Par-4 gene up regulation on K562 biological behaviour such as cell proliferation, apoptosis and cell cycle.

Methods Based on PC12 cell apoptosis induced by H_2O_2, cell survival rate was determined by MTr colorimetric analysis,prostate apoptosis response-4(Par-4)mRNA ex- pression and nuclear factor-kappa B(NF-κB)mRNA expression determined by reverse transcription-polymerase chain reaction(RT-PCR),Par-4 and NF-κB protein expression determined by Western blot method.

Objective: Through detecting the content of Par-4 protein and S100b protein in serum of neonates with hypoxic is chemic encephalopathy(HIE),to explore its clinical significance.

The present study constructed an eukaryotic expression recombinant plasmid named pEGFP-Brn-4 and observed its expression in mesenchymal stem cells(MSC).

Objective To investigate into epidemiologic characteristic and to study association between CTLA-4 genes and insulin dependent diabetes mellitus (IDDM) in children in Ningxia.