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1) SDS-binding method 点击朗读

SDS结合法

2) SDS method 点击朗读

SDS法

aconiti by using SDS method,CTAB method and the modified CTAB method resp. 点击朗读

[方法]以中药材附子药源植物的幼嫩叶片和块根为试材,分别采用SDS法、CTAB法和改良CTAB法从中药材附子药源植物中提取基因组DNA,比较不同方法的提取效果。

This research reported a development SDS method for extraction cotton genomic DNA. 点击朗读

通过在目前常用的SDS法的DNA提取液中加入抗氧化剂(PVP40和β-巯基乙醇)排除棉酚等次生物质干扰,进行了棉花基因组DNA的提取方法研究,结果表明,改良后的SDS法能够解决棉花DNA提取质量不高且容易褐变等问题。

To probe the adaptability of modified SDS method in apricot genomic DNA extraction and the optimal concentration, the genomic DNA samples of 6 apricot cultivars were isolated by 0.

为探讨改良SDS法是否适用于杏基因组DNA提取及提取液适宜浓度,以6个品种杏叶片为试材提取DNA,提取液设0。

更多例句>>

3) SDS

SDS法

We adopted the phenol-chloroform-isopentanol, CTAB and SDS methods to extract DNA from Sinononacula constricta , Meretix linnaeus,Chlamys nobilis, Haliotis diversicolor and other shellfishes.

以缢蛏、美人蛏、文蛤、扇贝和鲍鱼等几种经济贝类为研究对象,分别用苯酚／氯仿／异戊醇法,CTAB法,SDS法等不同方法提取其基因组DNA,寻找适宜于研究对象基因组DNA提取的方法,结果表明,3种方法均可以从目标材料中提取到DNA,但苯酚／氯仿／异戊醇法无论从数量和质量上都不理想,而SDS和CTAB可根据具体情况选择使用。

The results showed that SDS-CTAB method was the best one,then SDS method,and CTAB method was not recommended.

以籽蒿(Artemisia sphaerocephala Krasch)种子为试验材料,采用SDS法、CTAB法及SDS-CTAB法进行基因组DNA提取。

The tota1 DNA was extracted from the bean,horse bean and soybean with methods of high salt,SDS and CTAB.

以菜豆、蚕豆、黄豆的幼嫩叶片及其种子为材料,采用高盐低pH值法、SDS法和改良CTAB法3种不同的DNA提取方法提取其总DNA,用紫外分光光度法和琼脂糖凝胶电泳检测DNA质量。

更多例句>>

4) sodium dodecylsulphate-polyacrylamide gel electrophoresis 点击朗读

SDS-PAGE法

Methods Gel filtration chromatography(GFC),sodium dodecylsulphate-polyacrylamide gel electrophoresis(SDS-PAGE) technique and matrix-assisted laser desorption/ionizationtime of flight mass spectrometry(MALDI-TOF-MS)were used to determine the relative molecular weight,respectively.

方法采用凝胶过滤层析法、SDS-PAGE法和基质辅助激光解吸附电离飞行时间质谱法(MALDI-TOF)三种方法测定一种新型蚯蚓脱氧核糖核酸酶(EWD2)的分子量。

5) borax-SDS 点击朗读

硼砂-SDS法

In order to isolate high quality RNA from young leaves of Pyrus ussuriensis Maxim,in which polyphenols and polysaccharide are rich,The authors used five methods as follows: Trizol reagent;improved Trizol reagent;Guanidine Thiocyanate;borax-CTAB;borax-SDS,and established the most suitable isolation method of Pyrus ussuriensis Maxim RNA through the rate of yield,purity,electrophoresis and RT-PCR.

为了从富含多糖和多酚等物质的观赏山梨幼叶中提取和分离出高质量的RNA,利用Trizol法、改良的Trizol法、异硫氰酸胍法、硼砂-CTAB法和硼砂-SDS法提取观赏山梨叶片总RNA。

6) improved SDS method 点击朗读

改良SDS法

Method: The improved CTAB method and improved SDS method were used to extract DNA. 点击朗读

方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。

Traditional SDS method was simplified and improved,improved SDS method was found,DNA which was extracted from root tips of peanut by improved SDS method was high quality and was satisfied for molecular biology research.

对传统的SDS法进行简化和优化,建立了改良SDS法,利用改良SDS法提取花生根尖DNA,效果较好,能够满足分子生物学研究需要。

更多例句>>

补充资料：SD

分子式C₁₀H₁₀N₄O₂S，分子量250.28。俗称SD。又称对氨基-N-2-嘧啶基苯磺酸胺。白色或微黄色结晶粉末。无臭。熔点252～256℃。在空气中稳定，光照时易变成深色，难溶于水，微溶于乙醇、丙酮和乙醚。易溶于稀无机酸和苛性碱溶液。能治疗肺炎球菌、溶血性链球菌、脑膜炎球菌等的感染。适宜于小儿用药。也常制成水溶性的钠盐，用作注射针剂。可由对乙酰氨基苯磺酰氯与2-氨基嘧啶反应，然后再经水解制得。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

参考词条

SDS/酚法优化SDS法 SDS酸酚法 SDS层次结构 KCl-SDS沉淀法 SDS-PAGE电泳法 SDS-蛋白酶法 SDS碱裂解法 SDS法DNA提取

SDS-CTAB法 EDTA-SDS法 SDS-LiCl法

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	您的位置：首页 -> 词典 -> SDS结合法 1) SDS-binding method SDS结合法 2) SDS method SDS法 1. aconiti by using SDS method,CTAB method and the modified CTAB method resp. [方法]以中药材附子药源植物的幼嫩叶片和块根为试材,分别采用SDS法、CTAB法和改良CTAB法从中药材附子药源植物中提取基因组DNA,比较不同方法的提取效果。 2. This research reported a development SDS method for extraction cotton genomic DNA. 通过在目前常用的SDS法的DNA提取液中加入抗氧化剂(PVP40和β-巯基乙醇)排除棉酚等次生物质干扰,进行了棉花基因组DNA的提取方法研究,结果表明,改良后的SDS法能够解决棉花DNA提取质量不高且容易褐变等问题。 3. To probe the adaptability of modified SDS method in apricot genomic DNA extraction and the optimal concentration, the genomic DNA samples of 6 apricot cultivars were isolated by 0. 为探讨改良SDS法是否适用于杏基因组DNA提取及提取液适宜浓度,以6个品种杏叶片为试材提取DNA,提取液设0。更多例句>> 3) SDS SDS法 1. We adopted the phenol-chloroform-isopentanol, CTAB and SDS methods to extract DNA from Sinononacula constricta , Meretix linnaeus,Chlamys nobilis, Haliotis diversicolor and other shellfishes. 以缢蛏、美人蛏、文蛤、扇贝和鲍鱼等几种经济贝类为研究对象,分别用苯酚／氯仿／异戊醇法,CTAB法,SDS法等不同方法提取其基因组DNA,寻找适宜于研究对象基因组DNA提取的方法,结果表明,3种方法均可以从目标材料中提取到DNA,但苯酚／氯仿／异戊醇法无论从数量和质量上都不理想,而SDS和CTAB可根据具体情况选择使用。 2. The results showed that SDS-CTAB method was the best one,then SDS method,and CTAB method was not recommended. 以籽蒿(Artemisia sphaerocephala Krasch)种子为试验材料,采用SDS法、CTAB法及SDS-CTAB法进行基因组DNA提取。 3. The tota1 DNA was extracted from the bean,horse bean and soybean with methods of high salt,SDS and CTAB. 以菜豆、蚕豆、黄豆的幼嫩叶片及其种子为材料,采用高盐低pH值法、SDS法和改良CTAB法3种不同的DNA提取方法提取其总DNA,用紫外分光光度法和琼脂糖凝胶电泳检测DNA质量。更多例句>> 4) sodium dodecylsulphate-polyacrylamide gel electrophoresis SDS-PAGE法 1. Methods Gel filtration chromatography(GFC),sodium dodecylsulphate-polyacrylamide gel electrophoresis(SDS-PAGE) technique and matrix-assisted laser desorption/ionizationtime of flight mass spectrometry(MALDI-TOF-MS)were used to determine the relative molecular weight,respectively. 方法采用凝胶过滤层析法、SDS-PAGE法和基质辅助激光解吸附电离飞行时间质谱法(MALDI-TOF)三种方法测定一种新型蚯蚓脱氧核糖核酸酶(EWD2)的分子量。 5) borax-SDS 硼砂-SDS法 1. In order to isolate high quality RNA from young leaves of Pyrus ussuriensis Maxim,in which polyphenols and polysaccharide are rich,The authors used five methods as follows: Trizol reagent;improved Trizol reagent;Guanidine Thiocyanate;borax-CTAB;borax-SDS,and established the most suitable isolation method of Pyrus ussuriensis Maxim RNA through the rate of yield,purity,electrophoresis and RT-PCR. 为了从富含多糖和多酚等物质的观赏山梨幼叶中提取和分离出高质量的RNA,利用Trizol法、改良的Trizol法、异硫氰酸胍法、硼砂-CTAB法和硼砂-SDS法提取观赏山梨叶片总RNA。 6) improved SDS method 改良SDS法 1. Method: The improved CTAB method and improved SDS method were used to extract DNA. 方法:采用改良CTAB法和改良SDS法提取澳洲坚果样品的总DNA,并对产物进行紫外、电泳及PCR扩增检测。 2. Traditional SDS method was simplified and improved,improved SDS method was found,DNA which was extracted from root tips of peanut by improved SDS method was high quality and was satisfied for molecular biology research. 对传统的SDS法进行简化和优化,建立了改良SDS法,利用改良SDS法提取花生根尖DNA,效果较好,能够满足分子生物学研究需要。更多例句>> 补充资料：SD 分子式C₁₀H₁₀N₄O₂S，分子量250.28。俗称SD。又称对氨基-N-2-嘧啶基苯磺酸胺。白色或微黄色结晶粉末。无臭。熔点252～256℃。在空气中稳定，光照时易变成深色，难溶于水，微溶于乙醇、丙酮和乙醚。易溶于稀无机酸和苛性碱溶液。能治疗肺炎球菌、溶血性链球菌、脑膜炎球菌等的感染。适宜于小儿用药。也常制成水溶性的钠盐，用作注射针剂。可由对乙酰氨基苯磺酰氯与2-氨基嘧啶反应，然后再经水解制得。说明：补充资料仅用于学习参考，请勿用于其它任何用途。参考词条 SDS/酚法优化SDS法 SDS酸酚法 SDS层次结构 KCl-SDS沉淀法 SDS-PAGE电泳法 SDS-蛋白酶法 SDS碱裂解法 SDS法DNA提取

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