1) mammary grand-specific expression
乳腺特异表达
1.
It is discribed that the restructuring carrier had succeeded in cloning the area of control casein gene 5′and 3′,the recombinant DNA technology had been used to subclone into the modified eukaryotic expression vector pcDNA3(removal of CMV promoter),verificated by the restriction enzyme digestion and sequence analysis,the mammary grand-specific expression vector of cattle had been constructed.
然后利用DNA重组技术依次亚克隆入改造过的真核表达载体pcD-NA3(切除CMV启动子),构建成牛乳腺特异表达载体。
2.
The mammary grand-specific expression vector of cattle had been constructed.
经测序鉴定后,利用DNA重组技术依次亚克隆入改造过的真核表达载体pcDNA3(切除CMV启动子),并插入人溶菌酶基因(hLYZ)的cD-NA,构建成牛乳腺特异表达载体。
3) mammary gland-specific expression vector
乳腺特异性表达载体
1.
In the research, the mammary gland-specific expression vector pBC1-TPA and pBC2-TPA were constructed, which were consisted of regulating elements regulation region of bovineβ-casein and expression sequence finger-domain lacking t-PA cDNA.
本研究用奶牛β-酪蛋白基因调控序列和人组织型纤溶酶原激活剂指形区缺失体(t-PA指形区缺失体)表达序列,构建了乳腺特异性表达载体pBC1-TPA和pBC2-TPA。
4) mammary gland specific expression vector
乳腺特异定位表达载体
5) mammary-tissue-specific expression
乳腺组织特异性表达
补充资料:乳腺
乳腺
指乳房内的腺叶。乳房内有15~20个以乳头为中心呈辐射状排列的腺叶。每个腺叶内含有腺泡及导管(输乳管),叶间由结缔组织间隔,导管开口于乳头。乳腺小叶间质中有纤维束,将胸腺管与胸肌筋膜及真皮紧密相连。
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