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1)  K562/MDR cells
K562/MDR细胞
1.
Methods K562/MDR cells in culture medium were treated with TMP(50—6 400 mg·L-1) and CsA(0.
L-1)作用于体外培养的K562/MDR细胞72 h,采用MTT法检测细胞生长率,确定TMP和CsA的非细胞毒性剂量;采用非细胞毒性剂量,实验分为5组:G1组(TMP+ADM+K562/MDR)、G2组(CsA+ADM+K562/MDR)、G3组(TMP+CsA+ADM+K562/MDR)、阴性对照组(以K562/S代替K562/MDR)、空白对照组(以1640培养基代替药物),检测各组细胞生长抑制50%的ADM浓度,即IC50,计算耐药倍数和逆转倍数;利用荧光分光光度法检测各组细胞内ADM浓度;流式细胞术检测跨膜糖蛋白P-gp的表达情况。
2)  K562 cell
K562细胞
1.
An experimental study on reconstruction of retrovirus vector in human leukemia K562 cell xenograft tumor of nude Mice;
重组逆转录病毒RV-40AS对K562细胞裸鼠移植瘤的实验研究
2.
Effect of total saponins of panax ginseng on the expression of erythropoietin receptor in K562 cells;
人参总皂苷对K562细胞红细胞生成素受体表达的影响
3.
Study on apoptosis of K562 cells staining with Annexin V/PI induced by high-voltage pulse electric field;
Annexin V/PI双标记检测高电压脉冲电场诱导K562细胞凋亡的研究
3)  K562 cell line
K562细胞株
1.
Cytotoxicity of cord blood monocyte-macrophage activated by cytokines against K562 cell line;
细胞因子激活的脐血单核细胞对K562细胞株杀伤作用的观察
2.
Aim To explore the effect of 4-amino-2-trifluoromethyl-phenyl retinate(ATPR)on proliferation,differentiation activity in K562 cell line,and to research the mechanisms.
目的本研究探讨新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯(4-amino-2-trifluoromethyl-phenyl retinate,ATPR)对K562细胞株的抑制增殖和诱导分化活性并对其机制进行研究。
4)  K562 cells
k562细胞
1.
Differentiation of K562 cells into DC-like cells induced by the combination of calcium ionorphore with rhGM-CSF and rhIL-4;
钙离子载体促进K562细胞诱导分化成DC样细胞的研究
2.
Anti-ABL tyrosine kinase intrabody inhibits growth of K562 cells in vitro;
抗ABL酪氨酸激酶区胞内抗体抑制K562细胞体外生长
3.
Effects of the main extracts of Astragalus membranaceus on inducing the erythroid differentiation of K562 cells;
黄芪不同提取物对K562细胞向红系分化的诱导作用研究
5)  K562 cell line
K562细胞
1.
Effects of ginsenoside-Rh_2 on proliferation and apoptosis for K562 cell line;
人参皂甙单体Rh_2对K562细胞增殖和凋亡作用的实验研究
2.
Effects of p53 gene on K562 cell line proliferation;
P53基因对K562细胞增殖的影响
3.
The proteins of K562 cell line were separated by three modes of chromatography,the chromatographic fractions were collected and then detected by MALDI-TOF MS.
分别通过3种色谱模式:反相高效液相色谱(RPLC)、弱阴离子交换-反相高效液相色谱(WAX-RPLC)和排阻-反相高效液相色谱(SEC-RPLC)对K562细胞的蛋白质进行分离,收集的色谱馏分采用基质辅助电离解析时间飞行质谱(MALDI-TOF MS)进行鉴定后,比较所获得的蛋白质数据。
6)  K562/ADM cells
K562/ADM细胞
1.
Objective To study the effect of siRNA on mdr1 expression and function in K562/ADM cells and explore a new reversal path of drug resistance.
方法:siRNA根椐GeneBank已知序列设计,在脂质体介导下转染K562/ADM细胞;用流式细胞仪检测K562/ADM细胞Pgp的表达及细胞周期的改变;用TUNEL法检测其凋亡;用MTTT检测其对阿霉素ADM的敏感性。
2.
OBJECTIVE To cluster analysis and optimally select the herbs with reversal activity of multi-drug resistance in K562/ADM cells.
方法选用补骨脂素、苦参碱、人参皂苷Rb1、槲皮素、姜黄素、贝母碱、川芎嗪、大黄酸8种中药成分,MTT比色法测定药物对K562/ADM细胞的增殖抑制作用,流式细胞仪测定细胞内化疗药物ADM荧光强度,测定细胞P糖蛋白表达;就测定的相关数据,通过多元统计中的聚类分析方法进行药物分组,以及采用模糊理论中的集值统计方法进行优选排序。
3.
Methods: After treatment of K562/ADM cells with cAMP(0.
目的:研究环腺苷酸(cyclicadenosinemonophosphate,cAMP)对人白血病多药耐药K562/ADM细胞的诱导分化作用。
补充资料:[styrene-(2-vinylpyridine)copolymer]
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性质:学名苯乙烯-2-乙烯吡啶共聚物。微黄色粉末或透明小颗粒晶体。无臭,无味。不溶于水,溶于酸、乙醇、丙酮、氯仿。有抗水、防潮性能,适用于多种药片的包衣等。

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