1) drug-resistance rpsL gene
耐药基因rpsL
1.
The genome DNA of the multi-drug resistant strains of clinical isolated Mycobacterium tuberculosis was prepared and the drug-resistance rpsL gene and rrS gene of the Mycobacterium tuberculosis were enlarged by PCR,and were sequenced.
制备多耐药临床分离株结核分支杆菌基因组DNA,PCR扩增其耐药基因rpsL基因和rrS基因,同时进行测序分析,结果显示该株多耐药临床分离株结核分支杆菌的rpsL基因的93、94bp处的碱基发生了突变,碱基C、C突变为T、T,使得相对应的编码氨基酸由脯氨酸(Pro)突变为亮氨酸(Leu);而rrS基因未发生核苷酸的插入、缺失或取代;进一步构建该株多耐药临床分离株结核分支杆菌耐药基因rpsL高效原核表达重组载体pGEX-λT/rpsL,所构建的重组质粒pGEX-λT/rpsL在大肠杆菌中高效表达了38kD的融合蛋白。
2) SM resistant Rpsl gene
耐链霉素Rpsl基因
3) rpsL
rpsL基因
1.
The genetic mutation of katG,rpoB and rpsL were detected by PCR-SSCP,and the results were compared with those by traditional antimicrobial susceptibility test(AST).
tuberculosis菌株32株,采用PCR-SSCP法检测katG、rpoB和rpsL基因突变,与采用常规药敏试验(AST)法检测的耐药结果进行对比分析。
2.
Objective To study the relationship between mutation of rpsL drug-resistant gene in L-forms of Mycobacterium tuberculosis and drug-resistance to streptomycin in pneumoconiosis patients.
方法用PCR-SSCP方法检测rpsL基因,与采用常规SM药敏试验(AST法)的结果进行对比分析。
3.
METHODS: katG, rpoB, rpsL genes mutation in 58 clinical isolated strains of Mycobacterium tuberculosis were detecting by PCR SSCP and traditional drug susceptibility test, and the results were compared.
方法 :用PCR SSCP方法检测 5 8株结核分枝杆菌临床分离株katG ,rpoB ,rpsL基因突变并与常规药敏试验检测结果进行对比 。
4) RpsL gene
rpsL基因
1.
Objective:To evaluate the value of detection of Rpob、KatG and rpsL gene mutation in multi-drug resistant clinical iso- lating strain of Mycobacterium tuberculosis in patients with diabetes and pulmonary tuberculosis.
目的:探讨耐多药结核分枝杆菌临床分离株rPOB、KatG和rpsL基因突变在糖尿病合并肺结核患者耐药性检测中的应用价值。
2.
Objective: The present study is to report the KatG, rpoB, rpsL genes mutation in 71clinical isolatedstrains of MTB in Yantai, and to analyze the relationship of genemutation and drug resistance inmycobacterium tuberculosis(MTB).
目的：本文采用PCR-SSCP银染法对烟台地区71例肺结核患者痰标本临床分离株同时进行KatG基因、rpoB基因和rpsL基因突变分析，探讨烟台地区结核分枝杆菌耐药基因的检测与耐药水平的关系，监测耐药菌株的发展，有助于指导临床治疗和预防。
3.
tuberculosis of 50 showed the mutation in rpsL genes.
PCR-LiPA方法分析分析结果:耐SM菌株中7株rpsL基因突变、SM敏感株1株rpsl基因突变,rrs基因未发现突变;与DNA测序结果的符合率为100%。
5) Resistant genes
耐药基因
1.
Detection of resistence to erythromycin and related resistant genes in streptococcus pneumoniae strains;
儿童肺炎链球菌分离株红霉素耐药性与相关耐药基因研究
2.
Analysis of enterococcal antimicrobial resistance and detection on antibiotics-resistant genes in children
儿童肠球菌耐药性分析和部分耐药基因检测
3.
Resistant genes encoding β-lactamases,aminoglycoside modifying enzymes and antiseptic resistance were detected by PCR amplification and verified by DNA sequencer.
结论临床分离的铜绿假单胞菌已携带多种耐药基因。
6) resistance gene
耐药基因
1.
Analysis of drug resistance genes in extended spectrum β-lactamases producing Escherichia coli;
产超广谱β-内酰胺酶大肠埃希菌耐药基因分析
2.
Disinfectant-sulfadiazine Resistance genes in Escherichia coli Isolated from Elderly Wards
老年病区大肠埃希菌分离株消毒剂-磺胺耐药基因携带的研究
3.
Objective: To understand the resistance gene spectrum and genetics characteristics of Klebsiella pneumoniae(Kpn) producting ESBLs.
目的:了解产ESBLs肺炎克雷伯菌的耐药基因谱及其遗传学特征。
补充资料：抗药基因
分子式：
CAS号：
性质：赋予宿主细胞对抗菌物质有耐受性的一类基因。通常由基因突变产生，如链霉素能与敏感菌株的核蛋白体30S小亚基结合，抑制细菌蛋白质合成。但是接触一定时间后，可因基因突变产生结构改变了的核蛋白体，使链霉素不能与之结合而失效。抗药基因还包括质粒所携带的抗药基因，常常是为特异分解此药物的酶编码的基因。带有抗药基因的质粒也称R因子或R质粒。R因子除能遗传子代外，还能通过噬菌体转导和细菌间的接合传布而产生新的耐药菌株。此外，耐药菌株溶解后释出的DNA还可重组进入敏感菌染色质中。
CAS号：
性质：赋予宿主细胞对抗菌物质有耐受性的一类基因。通常由基因突变产生，如链霉素能与敏感菌株的核蛋白体30S小亚基结合，抑制细菌蛋白质合成。但是接触一定时间后，可因基因突变产生结构改变了的核蛋白体，使链霉素不能与之结合而失效。抗药基因还包括质粒所携带的抗药基因，常常是为特异分解此药物的酶编码的基因。带有抗药基因的质粒也称R因子或R质粒。R因子除能遗传子代外，还能通过噬菌体转导和细菌间的接合传布而产生新的耐药菌株。此外，耐药菌株溶解后释出的DNA还可重组进入敏感菌染色质中。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条